Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis.

Diagnosing Melioidosis System Real-Time PCR Assay for Clinical Evaluation of a Type III Secretion

Development and evaluation of a real-time PCR assay targeting the type III secretion system of Burkholderia pseudomallei.

Here we report on the development of a discriminatory real-time assay for the rapid identification of Burkholderia pseudomallei isolates and the evaluation of this assay for sensitivity against related species and detection in spiked human blood samples. The assay targets a 115-base-pair region within orf2 of the B. pseudomallei type III secretion system gene cluster and distinguishes B. pseudo...

Analysis of Herpes Simplex Virus in Suspected Encephalitis, Keratitis and Dermal Infections Using Real- Time PCR

Background & Objectives: Herpes viruses can cause diseases in the clinical range. The virus can cause infection in various body parts, especially eyes and nervous system. The aim of this study was at evaluating  the Real-Time TaqMan probe PCR in diagnosing and monitoring of the patients with suspected HSV infections.Materials & Methods: More than a thousand patients with suspected HSV infection...

Evaluation of a new set of Real-Time PCR for Brucella detection within human and animal samples

A quantitative TaqMn Real-Time PCR assay was developed and its diagnostic value on human serum and livestock samples were evaluated. Brucella species could be distributed through communities as a biological agent. Rapid detection of biological threat agents is critical for timely therapeutic administration. Quantitative real-time PCR provides a rapid, sensitive and specific tool for molecular i...

Comparison of DNA Extraction Kits for Detection of Burkholderia pseudomallei in Spiked Human Whole Blood Using Real-Time PCR

Burkholderia pseudomallei, the etiologic agent of melioidosis, is endemic in northern Australia and Southeast Asia and can cause severe septicemia that may lead to death in 20% to 50% of cases. Rapid detection of B. pseudomallei infection is crucial for timely treatment of septic patients. This study evaluated seven commercially available DNA extraction kits to determine the relative recovery o...